RESUMO
BACKGROUND: Application of chemotherapy to treatment of parasitic disease of man and animals can be problematic due to different adverse effects. As a result, there is an increasing interest in nanoparticles as new therapeutic tools against these diseases. This study was designed to evaluate the effect of selenium and copper oxide nanoparticles on Giardia deudenalis cysts in vitro, as well as comparing it to that of metronidazole. METHODS: The cysts were taken from the stools of patients in Urmia, Iran, during 2017-2018. The cysts were taken from stool and were concentrated and isolated on 0.85 M-sucrose. Then, selenium and copper oxide nanoparticles were prepared at concentrations of 0.15, 0.3, and 0.6 mg/ml. The effect of nanoparticle's various concentrations at 10, 15, 30, 60, and 180 min were evaluated and compared to control groups. Obtained data was recorded and statistically analyzed. RESULTS: Copper oxide nanoparticles at a concentration of 0.6 mg/ml and selenium nanoparticles at a concentration of 0.3 mg/ml had the same effect as of metronidazole in killing of Giardia cysts. The cytotoxic effects of selenium and copper oxide nanoparticles, compared with metronidazole, on Giardia cysts, showed an increase of fatality rate due to extend exposure time and nanoparticle's concentration (P<0.05). CONCLUSION: Selenium and copper oxide nanoparticles are as efficient as metronidazole, for killing Giardia cysts in vitro.
